Structure and transcription of the Drosophila melanogaster vermilion gene and several mutant alleles

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Molecular analysis of mutations induced in the vermilion gene of Drosophila melanogaster by methyl methanesulfonate.

The nature of DNA sequence changes induced by methyl methanesulfonate (MMS) at the vermilion locus of Drosophila melanogaster was determined after exposure of postmeiotic male germ cell stages. MMS is a carcinogen with strong preference for base nitrogen alkylation (s = 0.86). The spectrum of 40 intralocus mutations was dominated by AT----GC transitions (23%), AT----TA transversions (54%) and d...

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Splicing of retrotransposon insertions from transcripts of the Drosophila melanogaster vermilion gene in a revertant.

A mutation of the Drosophila melanogaster vermilion (v) gene known as v1 is caused by the insertion of a 412 retrotransposon into the 5' untranslated region of the first exon. Mutants carrying this insertion accumulate a low level of mRNA from which most of the transposon sequences have been eliminated by splicing at cryptic sites within transposon sequences. Here, we demonstrate that a reverta...

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ژورنال

عنوان ژورنال: Molecular and Cellular Biology

سال: 1990

ISSN: 0270-7306,1098-5549

DOI: 10.1128/mcb.10.4.1423-1431.1990